TB-500 for dogs: dosage chart by weight

TB-500 is sold as a synthetic fragment of thymosin beta-4, a naturally occurring protein that moves repair cells to damaged tissue. Working-dog and equine circles have used it for recovery for well over a decade.

Canitide REPAIR · 5 days on / 2 days off · 8-week cycle, then 4 weeks off

TB-500 dose by weight band.

One pen unit is 0.01 mL and carries about 33 mcg of TB-500. The bands are set from the mg/kg ranges in the animal research, rounded to whole units.

Weight band	Per dose	Per dose (mcg)	Per week	Cartridge lasts	Club cadence
Small (under 20 lb)	6 units	198 mcg	30 units	~10 weeks	every 8 weeks
Medium (20–50 lb)	9 units	297 mcg	45 units	~6 weeks	every 6 weeks
Large (50–90 lb)	12 units	396 mcg	60 units	~5 weeks	every 4 weeks
Giant (90 lb and up)	15 units	495 mcg	75 units	~4 weeks	every 4 weeks

Cartridges hold 300 usable units after the 2-unit prime. Missed a dose? Skip it and continue; never double up.

Download this chart (PDF) ↓

Canitide products are sold for research purposes and are not FDA-approved drugs. Talk to your veterinarian before starting any protocol, and bring the dosing card.

Your dog

Enter a weight, get the number.

It is almost always run alongside BPC-157, which is why Canitide sells the pair pre-mixed in one REPAIR cartridge rather than two vials. The dose per weight band is the same number of units for both peptides.

Reserve Canitide REPAIR

Your dog’s weight

If you use a vial

A 10 mg vial of TB-500 in 3 mL of bacteriostatic water is 3.33 mg/mL, the same concentration as the TB-500 in a REPAIR cartridge. On a U-100 insulin syringe one unit is also 0.01 mL, so the chart above reads the same. What changes with the pen: no reconstitution, no drawing up, a sterility-tested cartridge, a 32G × 4 mm needle, and a dial you cannot misread. The pen versus vial kits.

Questions about TB-500 for dogs

What is the TB-500 dose for dogs per pound?

Canitide does not dose per pound; it doses by band, because the animal research is in mg/kg ranges and a pen dials whole units. REPAIR gives 6, 9, 12 or 15 units a dose for dogs under 20 lb, 20–50 lb, 50–90 lb and 90 lb and up. Each unit carries about 33 mcg of TB-500 and 33 mcg of BPC-157.

Can I give BPC-157 and TB-500 together?

Yes; that is the usual pairing in working-dog recovery and the reason REPAIR is one cartridge. The working theory is that BPC-157 acts locally on blood supply and tendon cells while the thymosin fragment supports repair-cell movement. The theory comes from separate studies, not a trial of the combination.

How long is a TB-500 cycle?

REPAIR runs five days on, two days off, for eight weeks, then four weeks off. Many owners then run maintenance cycles.

What does the research say?

Thymosin beta-4 has been shown, in mouse models, to promote cell migration and cardiac repair. A 2012 analytical study characterized the acetylated seven-residue fragment in a tested preparation. There is no controlled trial in dogs.

The research

Primary sources only, each labeled with the model it was studied in.

rat tendonStable gastric pentadecapeptide BPC 157 accelerates healing of transected rat Achilles tendonStaresinic M et al. · J Orthop Res 2003
rat tendon fibroblastsThe promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migrationChang CH et al. · J Appl Physiol 2011
reviewBrain-gut axis and pentadecapeptide BPC 157: theoretical and practical implicationsSikiric P et al. · Curr Neuropharmacol 2016
reviewThymosin β4: actin-sequestering protein moonlights to repair injured tissuesGoldstein AL, Hannappel E, Kleinman HK · Trends Mol Med 2005
mouseThymosin β4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repairBock-Marquette I et al. · Nature 2004
dogs (epidemiology)Prevalence of radiographic appendicular osteoarthritis and associated clinical signs in young dogsEnomoto M et al. · Sci Rep 2024

All sources